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LearnMA5-31846
antibody from Invitrogen Antibodies
Targeting: NLRC4
CARD12, CLAN, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, ipaf
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [2]
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- Product number
- MA5-31846 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-NLRC4 (Ser533) Monoclonal Antibody (4B7B7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 4B7B7
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-NLRC4 (Ser533) in NIH/3T3 (1) cell lysate. Sample was incubated with Phospho-NLRC4 (Ser533) monoclonal antibody (Product # MA5-31846) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of Phospho-NLRC4 (Ser533) in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with Phospho-NLRC4 (Ser533) monoclonal antibody (Product # MA5-31846) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Phospho-NLRC4 (Ser533) in HeLa cells. Sample was incubated with Phospho-NLRC4 (Ser533) monoclonal antibody (Product # MA5-31846) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Phospho-NLRC4 (Ser533) in Hela cells (green). Sample was incubated with Phospho-NLRC4 (Ser533) monoclonal antibody (Product # MA5-31846) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
