Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-22151 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCM3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (98%), Rat (99%), Zebrafish (86%), Xenopus laevis (89%), Chicken (90%), Rhesus Monkey (100%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MCM3 Polyclonal Antibody (Product # PA5-22151). Sample (30 µg of whole cell lysate). Lane A: Hep G2 . 7.5% SDS PAGE. MCM3 Polyclonal Antibody (Product # PA5-22151) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MCM3 Polyclonal Antibody (Product # PA5-22151). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM3 Polyclonal Antibody (Product # PA5-22151) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MCM3 Polyclonal Antibody (Product # PA5-22151). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM3 Polyclonal Antibody (Product # PA5-22151) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCM3 in paraformaldehyde-fixed HeLa cells using a MCM3 polyclonal antibody (Product # PA5-22151) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCM3 Polyclonal Antibody detects MCM3 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. MCM3 Polyclonal Antibody (Product # PA5-22151) diluted at 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.