Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- H00001820-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001820-M01, RRID:AB_464155
- Product name
- ARID3A monoclonal antibody (M01), clone 1A11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant ARID3A.
- Antigen sequence
QYMKYLYPYECEKRGLSNPNELQAAIDSNRREGRR
QSFGGSLFAYSPGGAHGMLSSPKLPVSSLGLAAST
NGSSITPAPKIKKEEDSAIPITVPGRLPVS- Isotype
- IgG
- Antibody clone number
- 1A11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Expression profiling of more than 3500 proteins of MSS-type colorectal cancer by stable isotope labeling and mass spectrometry.
Kang UB, Yeom J, Kim HJ, Kim H, Lee C
Journal of proteomics 2012 Jun 6;75(10):3050-62
Journal of proteomics 2012 Jun 6;75(10):3050-62
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ARID3A monoclonal antibody (M01), clone 1A11 Western Blot analysis of ARID3A expression in K-562 ( Cat # L009V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ARID3A is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol