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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28294 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DRIL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, BCL-1.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DRIL1 Polyclonal Antibody detects ARID3A protein by Western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract.7.5 % SDS-PAGE. DRIL1 Polyclonal Antibody (Product # PA5-28294) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DRIL1 Polyclonal Antibody detects ARID3A protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract.7.5 % SDS-PAGE. DRIL1 Polyclonal Antibody (Product # PA5-28294) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DRIL1 in paraformaldehyde-fixed HeLa cells using a DRIL1 polyclonal antibody (Product # PA5-28294) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ARID3A (Product # PA5-28294) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DRIL1 antibody immunoprecipitates DRIL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of DRIL1 protein by 2 µg of DRIL1 antibody (Product # PA5-28294) 7.5% SDS-PAGE The immunoprecipitated DRIL1 protein was detected by DRIL1 antibody (Product # PA5-28294) diluted at 1:1,000.
