PA5-35849

antibody from Invitrogen Antibodies

Targeting: AGMAT FLJ23384

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35849

Provider

Invitrogen Antibodies

Product name

AGMAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, mouse liver, rat liver.

Concentration

0.6 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AGMAT was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AGMAT Polyclonal Antibody (Product # PA5-35849) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AGMAT was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AGMAT Polyclonal Antibody (Product # PA5-35849) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AGMAT was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AGMAT Polyclonal Antibody (Product # PA5-35849) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AGMAT Polyclonal Antibody detects Agmatinase protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: Agmatinase protein stained by AGMAT Polyclonal Antibody (Product # PA5-35849) diluted at 1:500. Blue: Hoechst 33342 staining.