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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX111919 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111919, RRID:AB_1949952
- Product name
- CDK7 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Regulation of Transcription Elongation by the XPG-TFIIH Complex Is Implicated in Cockayne Syndrome.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Regulation of Transcription Elongation by the XPG-TFIIH Complex Is Implicated in Cockayne Syndrome.
Narita T, Narita K, Takedachi A, Saijo M, Tanaka K
Molecular and cellular biology 2015 Sep;35(18):3178-88
Molecular and cellular biology 2015 Sep;35(18):3178-88
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDK7 antibody detects CDK7 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g A375 whole cell lysate/extract10% SDS-PAGECDK7 antibody (GTX111919) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDK7 antibody detects CDK7 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract C. 30 ?g C8D30 whole cell lysate/extract D. 30 ?g NIH-3T3 whole cell lysate/extract E. 30 ?g BCL-1 whole cell lysate/extract F. 30 ?g Raw264.7 whole cell lysate/extract G. 30 ?g C2C12 whole cell lysate/extract 10% SDS-PAGECDK7 antibody (GTX111919) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDK7 antibody immunoprecipitates CDK7 protein in IP experiments.IP samples: 293T whole cell extractA. 40 £gg 293T whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of CDK7 protein by 4 £gg CDK7 antibody (GTX111919)10 % SDS-PAGEThe immunoprecipitated CDK7 protein was detected by CDK7 antibody (GTX111919) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using CDK7(GTX111919) antibody at 1:250 dilution.
