MA3-001

antibody from Invitrogen Antibodies

Targeting: CDK7 CAK, CAK1, CDKN7, MO15, STK1

Antibody data

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Validation data

Product number: MA3-001 - Provider product page
Provider: Invitrogen Antibodies
Product name: CDK7 Monoclonal Antibody (31TF2-1F8)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: MA3-001 has been used successfully in Western blotting to detect Cyclin dependent kinase 7 in human lysates.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 31TF2-1F8
Vial size: 50 µL
Concentration: Conc. Not Determined
Storage: -20° C, Avoid Freeze/Thaw Cycles

CDK7 protein structure - MA3-001 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CDK7 was performed by loading 50 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a PVDF membrane using the G2 Blotter (Product # 62288), and blocked with Starting Block T20 (Product # 37543) for 1 hour at room temperature. CDK7 was detected at ~38 kD using a CDK7 monoclonal antibody (Product # MA3-001) at a dilution of 1:1000 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # 31430) at a dilution of 1:20,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product # 34078) and the myECL Imager (Product # 62236).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CDK7 was performed by loading 50 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a PVDF membrane using the G2 Blotter (Product # 62288), and blocked with Starting Block T20 (Product # 37543) for 1 hour at room temperature. CDK7 was detected at ~38 kD using a CDK7 monoclonal antibody (Product # MA3-001) at a dilution of 1:1000 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # 31430) at a dilution of 1:20,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product # 34078) and the myECL Imager (Product # 62236).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CDK7 was achieved by transfecting A-431 with CDK7 specific siRNAs (Silencer® select Product # s2830). Western blot analysis (Fig. a) was performed using whole cell extracts from the CDK7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CDK7 Monoclonal Antibody (31TF2-1F8) (Product # MA3-001, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CDK7.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-CDK7 Monoclonal Antibody (31TF2-1F8) (Product # MA3-001) and a 39 kDa band corresponding to CDK7 was observed across cell lines and tissue extracts tested along with a 25kDa band which corresponds to free IgG. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), MCF-7 (Lane 3), HeLa (Lane 4), Jurkat (Lane 5), HCT116 (Lane 6), K-562 (Lane 7), tissue extracts of Mouse Heart (Lane 8), Mouse Testis (Lane 9) and Mouse Ovary (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution), 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).