Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- ABIN394716 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-AF4/FMR2 Family, Member 4 (AFF4) (AA 1-109) antibody
- Antibody type
- Monoclonal
- Description
- This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 1-109
- Isotype
- IgG
- Antibody clone number
- 2E12
- Vial size
- 100 μg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Submitted references HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia.
MCEF is localized to the nucleus by protein sequences encoded within three distinct exons, where it represses HIV-1 Tat-transactivation of LTR-directed transcription.
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.
He N, Liu M, Hsu J, Xue Y, Chou S, Burlingame A, Krogan NJ, Alber T, Zhou Q
Molecular cell 2010 May 14;38(3):428-38
Molecular cell 2010 May 14;38(3):428-38
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
Rose JE, Behm FM, Drgon T, Johnson C, Uhl GR
Molecular medicine (Cambridge, Mass.) 2010 Jul-Aug;16(7-8):247-53
Molecular medicine (Cambridge, Mass.) 2010 Jul-Aug;16(7-8):247-53
AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia.
Lin C, Smith ER, Takahashi H, Lai KC, Martin-Brown S, Florens L, Washburn MP, Conaway JW, Conaway RC, Shilatifard A
Molecular cell 2010 Feb 12;37(3):429-37
Molecular cell 2010 Feb 12;37(3):429-37
MCEF is localized to the nucleus by protein sequences encoded within three distinct exons, where it represses HIV-1 Tat-transactivation of LTR-directed transcription.
Niedzielski MF, Hopewell R, Ismail Z, Estable MC
International journal of biological sciences 2007 Mar 1;3(4):225-36
International journal of biological sciences 2007 Mar 1;3(4):225-36
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.
Jeronimo C, Forget D, Bouchard A, Li Q, Chua G, Poitras C, Thérien C, Bergeron D, Bourassa S, Greenblatt J, Chabot B, Poirier GG, Hughes TR, Blanchette M, Price DH, Coulombe B
Molecular cell 2007 Jul 20;27(2):262-74
Molecular cell 2007 Jul 20;27(2):262-74
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Supportive validation
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- WB
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- WB
Supportive validation
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- ELISA
Supportive validation
- Submitted by
- antibodies-online (provider)
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- Experimental details
- IHC