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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28971 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEMA7A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.82 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Antibody-based cell-surface proteome profiling of metastatic breast cancer primary explants and cell lines.
Donnenberg VS, Zhang JJ, Moravcikova E, Meyer EM, Lu H, Carson CT, Donnenberg AD
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2018 Apr;93(4):448-457
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2018 Apr;93(4):448-457
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD108/SEMA7A using 30 µg of A) A431 (B) H1299 and C) HeLa S3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CD108/SEMA7A polyclonal antibody (Product # PA5-28971) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SEMA7A was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SEMA7A Polyclonal Antibody (Product # PA5-28971) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SEMA7A was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SEMA7A Polyclonal Antibody (Product # PA5-28971) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using CD108 (Product # PA5-28971) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
