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ImmunocytochemistryAntibody data
- Antibody Data
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- Validations
- Western blot [8]
- Immunohistochemistry [3]
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- Product number
- NBP1-33061 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33061, RRID:AB_2245684
- Product name
- Rabbit Polyclonal DDB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDB1 Antibody [NBP1-33061] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDB1 Antibody [NBP1-33061] - Sample (30 ug of whole cell lysate) NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDB1 Antibody [NBP1-33061] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of DDB1 protein by 2. 5 ug of DDB1 antibody 7. 5% SDS-PAGE gel.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDB1 Antibody [NBP1-33061] - Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDB1 Antibody [NBP1-33061] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDB1 Antibody [NBP1-33061] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Paraffin-embedded Cal27 Xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Paraffin-embedded mouse prostate. DDB1 antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Paraffin-embedded rat fore brain. DDB1 antibody dilution: 1:500.
