H00079009-B01

antibody from Abnova Corporation

Targeting: DDX50 GU2, GUB, MGC3199, RH-II/GuB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00079009-B01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00079009-B01, RRID:AB_1113509

Product name

DDX50 MaxPab mouse polyclonal antibody (B01)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human DDX50 protein.

Antigen sequence

MPGKLLWGDIMELEAPLEESESQKKERQKSDRRKS
RHHYDSDEKSETRENGVTDDLDAPKAKKSKMKEKL
NGDTEEGFNRLSDEFSKSHKSRRKDLPNGDIDEYE
KKSKRVSSLDTSTHKSSDNKLEETLTREQKEGAFS
NFPISEETIKLLKGRGVTYLFPIQVKTFGPVYEGK
DLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSR
SPKVLVLAPTRELANQVAKDFKDITRKLSVACFYG
GTSYQSQINHIRNGIDILVGTPGRIKDHLQSGRLD
LSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTD
SEDNPQTLLFSATCPQWVYKVAKKYMKSRYEQVDL
VGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVY
SGSEGRAIIFCETKKNVTEMAMNPHIKQNAQCLHG
DIAQSQREITLKGFREGSFKVLVATNVAARGLDIP
EVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICIC
FYQPRERGQLRYVEQKAGITFKRVGVPSTMDLVKS
KSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVD
ALAAALAHISGASSFEPRSLITSDKGFVTMTLESL
EEIQDVSCAWKELNRKLSSNAVSQITRMCLLKGNM
GVCFDVPTTESERLQAEWHDSDWILSVPAKLPEIE
EYYDGNTSSNSRQRSGWSSGRSGRSGRSGGRSGGR
SGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRS
FD


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DDX50 expression in transfected 293T cell line (H00079009-T01) by DDX50 MaxPab polyclonal antibody.Lane 1: DDX50 transfected lysate(81.07 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to DDX50 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol