M00215-1

antibody from Boster Biological Technology

Targeting: SNCA NACP, PARK1, PARK4, PD1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00215-1

Provider

Boster Biological Technology

Product name

Anti-Alpha Synuclein SNCA Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for ALPHA SYNUCLEIN/SNCA detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human ALPHA SYNUCLEIN/SNCA information: Molecular Weight: 14460 MW; Subcellular Localization: Cytoplasm, cytosol . Membrane . Nucleus . Cell junction, synapse . Secreted . Membrane-bound in dopaminergic neurons; Tissue Specificity: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.

Reactivity

Human

Host

Rabbit

Antibody clone number

AGA-19

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse testis, using Alpha Synuclein Antibody(M00215-1)SNCA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SNCA Antibody (M00215-1)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image