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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- AF1338 - Provider product page
- Provider
- R&D Systems
- Product name
- Human alpha-Synuclein Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human alpha-Synuclein in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) beta-Synuclein and rh gamma-Synuclein is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P37840- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mitochondrial localization of alpha-synuclein protein in alpha-synuclein overexpressing cells.
Gene expression changes in postmortem tissue from the rostral pons of multiple system atrophy patients.
Shavali S, Brown-Borg HM, Ebadi M, Porter J
Neuroscience letters 2008 Jul 11;439(2):125-8
Neuroscience letters 2008 Jul 11;439(2):125-8
Gene expression changes in postmortem tissue from the rostral pons of multiple system atrophy patients.
Langerveld AJ, Mihalko D, DeLong C, Walburn J, Ide CF
Movement disorders : official journal of the Movement Disorder Society 2007 Apr 30;22(6):766-77
Movement disorders : official journal of the Movement Disorder Society 2007 Apr 30;22(6):766-77
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human alpha-Synuclein by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue, human brain (cerebellum) tissue, SH-SY5Y human neuroblastoma cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human alpha-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1338) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for alpha-Synuclein at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human alpha-Synuclein ELISA Standard Curve. Recombinant Human alpha-Synuclein protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha-Synuclein Monoclonal Antibody(Catalog # MAB13384) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1338) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- alpha-Synuclein in Human Brain. alpha-Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Goat Anti-Human alpha-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1338) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
