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LearnH00005307-M01
antibody from Novus Biologicals
Targeting: PITX1
BFT, POTX, PTX1
Western blot ELISA
Immunoprecipitation Immunohistochemistry Blocking/Neutralizing Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
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- Product number
- H00005307-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005307-M01, RRID:AB_547063
- Product name
- Mouse Monoclonal PITX1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PITX1 - paired-like homeodomain transcription factor 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Downregulated PITX1 Modulated by MiR-19a-3p Promotes Cell Malignancy and Predicts a Poor Prognosis of Gastric Cancer by Affecting Transcriptionally Activated PDCD5.
The c-Abl tyrosine kinase stabilizes Pitx1 in the apoptotic response to DNA damage.
Transcriptional activation of p53 by Pitx1.
Qiao F, Gong P, Song Y, Shen X, Su X, Li Y, Wu H, Zhao Z, Fan H
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(6):2215-2231
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(6):2215-2231
The c-Abl tyrosine kinase stabilizes Pitx1 in the apoptotic response to DNA damage.
Yamaguchi T, Miki Y, Yoshida K
Apoptosis : an international journal on programmed cell death 2010 Aug;15(8):927-35
Apoptosis : an international journal on programmed cell death 2010 Aug;15(8):927-35
Transcriptional activation of p53 by Pitx1.
Liu DX, Lobie PE
Cell death and differentiation 2007 Nov;14(11):1893-907
Cell death and differentiation 2007 Nov;14(11):1893-907
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - Analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi ( Cat # H00005307-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PITX1 monoclonal antibody (M01), clone 5G4 (Cat # H00005307-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - Analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody (M01), clone 5G4.Lane 1: PITX1 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - PITX1 monoclonal antibody (M01), clone 5G4 Analysis of PITX1 expression in Hela S3 NE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PITX1 Antibody (5G4) [H00005307-M01] - Analysis of PITX1 transfected lysate using anti-PITX1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PITX1 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PITX1 Antibody (5G4) [H00005307-M01] - Detection limit for recombinant GST tagged PITX1 is approximately 0.03ng/ml as a capture antibody.
