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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB1684 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1684, RRID:AB_1238008
- Product name
- AMFR polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of AMFR.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of an upstream region that controls the transcription of the human autocrine motility factor receptor.
Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor.
Huang B, Xie Y, Raz A
Biochemical and biophysical research communications 1995 Jul 26;212(3):727-42
Biochemical and biophysical research communications 1995 Jul 26;212(3):727-42
Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor.
Watanabe H, Carmi P, Hogan V, Raz T, Silletti S, Nabi IR, Raz A
The Journal of biological chemistry 1991 Jul 15;266(20):13442-8
The Journal of biological chemistry 1991 Jul 15;266(20):13442-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of AMFR (arrow) using AMFR polyclonal antibody (Cat # PAB1684). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AMFR gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with AMFR polyclonal antibody (Cat # PAB1684) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using AMFR polyclonal antibody (Cat # PAB1684)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
