MA5-29245

antibody from Invitrogen Antibodies

Targeting: ENTPD5 CD39L4, NTPDase-5, PCPH

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29245

Provider

Invitrogen Antibodies

Product name

ENTPD5 Recombinant Rabbit Monoclonal Antibody (64)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

64

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ENTPD5 in Lane A: HepG2 Whole Cell Lysate (30 µg). Samples were probed using a ENTPD5 Monoclonal Antibody (Product # MA5-29245) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:47 kDa. Observed band size:53 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 53 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ENTPD5 in human liver with ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245, 1:1,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ENTPD5 in human prostate with ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245, 1:1,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ENTPD5 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 0.5 µL with ENTPD5 Recombinant Rabbit Monoclonal Antibody (64) (Product # MA5-29245) and 15 µL of 50 % Protein G agarose. Primary antibody: ENTPD5 Recombinant Rabbit Monoclonal Antibody (64), at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa.