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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
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- Product number
- PA5-21700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MDH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat lung.
- Concentration
- 0.38 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MDH2 using 30 µg of 293T lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MDH2 polyclonal antibody (Product # PA5-21700) at a dilution of 1:1000.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of MDH2 was achieved by transfecting Hep G2 cells with MDH2 specific siRNAs (Silencer® select Product # s8624). Western blot analysis (Fig. a) was performed using whole cell extracts from the MDH2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with MDH2 Polyclonal Antibody (Product # PA5-21700, 1:3000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MDH2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MDH2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a MDH2 Polyclonal Antibody (Product # PA5-21700) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MDH2 Polyclonal Antibody (Product # PA5-21700). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein by western blot analysis. A. 50 µg rat lung lysate/extract.10% SDS-PAGE. MDH2 Polyclonal Antibody (Product # PA5-21700) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of C2C12 (Lane 1), L6 (Lane 2), Hep G2 (Lane 3), A-431 (Lane 4), LNCaP (Lane 5), tissue extracts Mouse Liver (Lane 6), Mouse Skeletal Muscle (Lane 7) and Rat Skeletal Muscle (Lane 8). The blot was probed with Anti-MDH2 Polyclonal Antibody (Product # PA5-21700, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 36 kDa band corresponding to MDH2 was observed across the cell lines and tissues tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MDH2 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. MDH2 Polyclonal Antibody (Product # PA5-21700) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MDH2 stained by MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. MDH2 stained by MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. MDH2 stained by MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:1,000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MDH2 stained by MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:1,000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MDH2 Polyclonal Antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. MDH2 Polyclonal Antibody (Product # PA5-21700) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
