PAB21328

antibody from Abnova Corporation

Targeting: GFRA3 GFRa-3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21328

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21328, RRID:AB_10963119

Product name

GFRA3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant GFRA3.

Antigen sequence

LPTESRLMNSCLQARRKCQADPTCSAAYHHLDSCT
SSISTPLPSEEPSVPADCLEAAQQLRNSSLIGCMC
HRRMKNQVACL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with GFRA3 polyclonal antibody (Cat # PAB21328) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with GFRA3 polyclonal antibody (Cat # PAB21328) at 1-4 ug/mL dilution shows positivity in plasma membrane and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lung with GFRA3 polyclonal antibody (Cat # PAB21328) shows strong positivity in alveolar cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)