Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF670 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GFR alpha-3/GDNF R alpha-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human GFR alpha-3/GDNF R alpha-3 in direct ELISAs and Western blots. In direct ELISA and Western blots, no cross-reactivity with recombinant human GFR alpha-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAC24355
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Functional mapping of receptor tyrosine kinases in myxoid liposarcoma.
Negri T, Virdis E, Brich S, Bozzi F, Tamborini E, Tarantino E, Jocollè G, Cassinelli G, Grosso F, Sanfilippo R, Casalini P, Greco A, Pierotti MA, Pilotti S
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jul 15;16(14):3581-93
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jul 15;16(14):3581-93
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human GFR alpha-3/GDNF R alpha-3 by Western Blot. Western blot shows lysates of human stomach tissue and human small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human GFR alpha-3/GDNF R alpha-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF670) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GFR alpha-3/GDNF R alpha-3 at approximately 55 and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.