Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-81671 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-81671, RRID:AB_1146576
- Product name
- Rabbit Polyclonal PF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex.
A PHD12-Snail2 repressive complex epigenetically mediates neural crest epithelial-to-mesenchymal transition.
Nishibuchi G, Shibata Y, Hayakawa T, Hayakawa N, Ohtani Y, Sinmyozu K, Tagami H, Nakayama J
The Journal of biological chemistry 2014 Oct 17;289(42):28956-70
The Journal of biological chemistry 2014 Oct 17;289(42):28956-70
A PHD12-Snail2 repressive complex epigenetically mediates neural crest epithelial-to-mesenchymal transition.
Strobl-Mazzulla PH, Bronner ME
The Journal of cell biology 2012 Sep 17;198(6):999-1010
The Journal of cell biology 2012 Sep 17;198(6):999-1010
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PF1 Antibody [NB100-81671] - Detection of Human and Mouse PF1 on HeLa whole cell lysate using NB100-81671. Pf1 was also immunoprecipitated by rabbit anti-PF1 antibody NB100-81670.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PF1 Antibody [NB100-81671] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PF1 Antibody [NB100-81671] - Detection of human PF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PF1 antibody NB100-81671 used for IP at 3 ug per reaction. PF1 was also immunoprecipitated by rabbit anti-PF1 antibody NB100-81670. For blotting immunoprecipitated PF1, NB100-81671 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.