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- Immunohistochemistry [3]
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- Product number
- NBP1-70342 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-70342, RRID:AB_11028745
- Product name
- Mouse Monoclonal SLC5A5/Sodium Iodide Symporter Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at approx 30 kDa, and approx 15kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Resveratrol inhibits sodium/iodide symporter gene expression and function in rat thyroid cells.
The flavonoid quercetin inhibits thyroid-restricted genes expression and thyroid function.
Giuliani C, Bucci I, Di Santo S, Rossi C, Grassadonia A, Mariotti M, Piantelli M, Monaco F, Napolitano G
PloS one 2014;9(9):e107936
PloS one 2014;9(9):e107936
The flavonoid quercetin inhibits thyroid-restricted genes expression and thyroid function.
Giuliani C, Bucci I, Di Santo S, Rossi C, Grassadonia A, Piantelli M, Monaco F, Napolitano G
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2014 Apr;66:23-9
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2014 Apr;66:23-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - analysis of Human thyroid lysate showing detection of Sodium Iodide Symporter protein using Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody, Clone 14F . Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Effects of 10 uM resveratrol on NIS protein expression. FRTL-5 cells were grown to 60% confluency in 6H5% medium, and then shifted to 5H5% medium for 6 days, before being cultured again in 6H5% medium for 24 h, and finally treated with resveratrol for 48 h. A representative Western blot is shown (top), with quantification (bottom). Data are the normalized means +/-SD (against actin) from three independent experiments (control vehicle: 100%). Control, cells treated with the control vehicle (0.5% ethanol); Resv, cells treated with 10 uM resveratrol. *, p
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Sodium Iodide Symporter Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
