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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX632360 - Provider product page
- Provider
- GeneTex
- Product name
- Filamin B antibody [GT1372]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT1372] (GTX632360) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Filamin B antibody [GT1372] detects Filamin B protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT1372] (GTX632360) at a dilution of 1:500.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT1372] (GTX632360) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Filamin B protein from HeLa whole cell extracts using 5 £gg of Filamin B antibody (GTX632360).Western blot analysis was performed using Filamin B antibody (GTX632360).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Filamin B antibody [GT1372] detects Filamin B protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Filamin B antibody [GT1372] (GTX632360) diluted at 1:250.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Filamin B antibody [GT1372] detects Filamin B protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Filamin B antibody [GT1372] (GTX632360) diluted at 1:250.
