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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3225 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3225, RRID:AB_1578779
- Product name
- PIK3R2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PIK3R2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Human immunodeficiency virus type 1 tat-mediated cytotoxicity of human brain microvascular endothelial cells.
HIV-1-Tat protein activates phosphatidylinositol 3-kinase/ AKT-dependent survival pathways in Kaposi's sarcoma cells.
Recruitment of phosphatidylinositol 3-kinase to CD28 inhibits HIV transcription by a Tat-dependent mechanism.
Khan NA, Di Cello F, Nath A, Kim KS
Journal of neurovirology 2003 Dec;9(6):584-93
Journal of neurovirology 2003 Dec;9(6):584-93
HIV-1-Tat protein activates phosphatidylinositol 3-kinase/ AKT-dependent survival pathways in Kaposi's sarcoma cells.
Deregibus MC, Cantaluppi V, Doublier S, Brizzi MF, Deambrosis I, Albini A, Camussi G
The Journal of biological chemistry 2002 Jul 12;277(28):25195-202
The Journal of biological chemistry 2002 Jul 12;277(28):25195-202
Recruitment of phosphatidylinositol 3-kinase to CD28 inhibits HIV transcription by a Tat-dependent mechanism.
Cook JA, August A, Henderson AJ
Journal of immunology (Baltimore, Md. : 1950) 2002 Jul 1;169(1):254-60
Journal of immunology (Baltimore, Md. : 1950) 2002 Jul 1;169(1):254-60
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3R2 polyclonal antibody (Cat # PAB3225) (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R2 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with PIK3R2 polyclonal antibody (Cat # PAB3225) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
