- NB100-1761 - Novus Biologicals MATR3 antibody

Submitted references Analysis of spinal and muscle pathology in transgenic mice overexpressing wild-type and ALS-linked mutant MATR3.

Moloney C, Rayaprolu S, Howard J, Fromholt S, Brown H, Collins M, Cabrera M, Duffy C, Siemienski Z, Miller D, Borchelt DR, Lewis J
Acta neuropathologica communications 2018 Dec 19;6(1):137

Matrin 3 variants are frequent in Italian ALS patients.

Marangi G, Lattante S, Doronzio PN, Conte A, Tasca G, Monforte M, Patanella AK, Bisogni G, Meleo E, La Spada S, Zollino M, Sabatelli M
Neurobiology of aging 2017 Jan;49:218.e1-218.e7

Heterogeneity of Matrin 3 in the developing and aging murine central nervous system.

Rayaprolu S, D'Alton S, Crosby K, Moloney C, Howard J, Duffy C, Cabrera M, Siemienski Z, Hernandez AR, Gallego-Iradi C, Borchelt DR, Lewis J
The Journal of comparative neurology 2016 Oct 1;524(14):2740-52

Transgenic mice overexpressing the ALS-linked protein Matrin 3 develop a profound muscle phenotype.

Moloney C, Rayaprolu S, Howard J, Fromholt S, Brown H, Collins M, Cabrera M, Duffy C, Siemienski Z, Miller D, Swanson MS, Notterpek L, Borchelt DR, Lewis J
Acta neuropathologica communications 2016 Nov 18;4(1):122

TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells.

Yarosh CA, Tapescu I, Thompson MG, Qiu J, Mallory MJ, Fu XD, Lynch KW
Nucleic acids research 2015 Oct 15;43(18):9006-16

The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition.

Moldovan JB, Moran JV
PLoS genetics 2015 May;11(5):e1005121

Disruption of the lamin A and matrin-3 interaction by myopathic LMNA mutations.

Depreux FF, Puckelwartz MJ, Augustynowicz A, Wolfgeher D, Labno CM, Pierre-Louis D, Cicka D, Kron SJ, Holaska J, McNally EM
Human molecular genetics 2015 Aug 1;24(15):4284-95

A novel nuclear FGF Receptor-1 partnership with retinoid and Nur receptors during developmental gene programming of embryonic stem cells.

Lee YW, Terranova C, Birkaya B, Narla S, Kehoe D, Parikh A, Dong S, Ratzka A, Brinkmann H, Aletta JM, Tzanakakis ES, Stachowiak EK, Claus P, Stachowiak MK
Journal of cellular biochemistry 2012 Sep;113(9):2920-36

Matrin 3 binds and stabilizes mRNA.

Salton M, Elkon R, Borodina T, Davydov A, Yaspo ML, Halperin E, Shiloh Y
PloS one 2011;6(8):e23882

The alphaherpesvirus US3/ORF66 protein kinases direct phosphorylation of the nuclear matrix protein matrin 3.

Erazo A, Yee MB, Banfield BW, Kinchington PR
Journal of virology 2011 Jan;85(1):568-81

Proteomic identification of FHL1 as the protein mutated in human reducing body myopathy.

Schessl J, Zou Y, McGrath MJ, Cowling BS, Maiti B, Chin SS, Sewry C, Battini R, Hu Y, Cottle DL, Rosenblatt M, Spruce L, Ganguly A, Kirschner J, Judkins AR, Golden JA, Goebel HH, Muntoni F, Flanigan KM, Mitchell CA, Bönnemann CG
The Journal of clinical investigation 2008 Mar;118(3):904-12

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Matrin 3 Antibody [NB100-1761] - The identification of host proteins immunoprecipitated with L1 ORF1p-FLAG. Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Primary antibodies used to probe western blots are indicated to the left of the images. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1005121) licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoprecipitation: Matrin 3 Antibody [NB100-1761] - IP of human fibroblasts. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoprecipitation: Matrin 3 Antibody [NB100-1761] - Detection of Human and Mouse Matrin 3 on HeLa whole cell lysate using NB100-1761. Matrin 3 was also immunoprecipitated using another rabbit anti-Matrin 3 antibody and NB100-1559.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Matrin 3 used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of NT transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (http://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of transverse sections from phenotypic Tg show an increase in Matrin 3 immunoreactivity compared to NT transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (http://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of f longitudinal sections from phenotypic Tg show an increase in Matrin 3 immunoreactivity compared to NT transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (http://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (http://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Matrin 3 Antibody [NB100-1761] - Section of human laryengeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Matrin 3 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

NB100-1761

Antibody data