H00002011-M01

antibody from Novus Biologicals

Targeting: MARK2 EMK1, PAR-1, PAR-1B, Par1b

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

H00002011-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00002011-M01, RRID:AB_2250358

Product name

Mouse Monoclonal MARK2 Antibody

Antibody type

Monoclonal

Description

IgG purified. MARK2 - MAP/microtubule affinity-regulating kinase 2

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

MARK2/Par1b kinase present at centrosomes and retraction fibres corrects spindle off-centring induced by actin disassembly.

Hart M, Zulkipli I, Shrestha RL, Dang D, Conti D, Gul P, Kujawiak I, Draviam VM
Open biology 2019 Jun 28;9(6):180263

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Spindle rotation in human cells is reliant on a MARK2-mediated equatorial spindle-centering mechanism.

Zulkipli I, Clark J, Hart M, Shrestha RL, Gul P, Dang D, Kasichiwin T, Kujawiak I, Sastry N, Draviam VM
The Journal of cell biology 2018 Sep 3;217(9):3057-3070

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Pancreatic LKB1 deletion leads to acinar polarity defects and cystic neoplasms.

Hezel AF, Gurumurthy S, Granot Z, Swisa A, Chu GC, Bailey G, Dor Y, Bardeesy N, Depinho RA
Molecular and cellular biology 2008 Apr;28(7):2414-25

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in PC-12.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: MARK2 Antibody (3B12) [H00002011-M01] - Detection limit for recombinant GST tagged MARK2 is approximately 0.03ng/ml as a capture antibody.