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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-22350 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCBP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji, mouse liver.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP E2 using 50 µg of mouse liver lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a hnRNP E2 polyclonal antibody (Product # PA5-22350) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCBP2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PCBP2 Polyclonal Antibody (Product # PA5-22350) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PCBP2 Polyclonal Antibody (Product # PA5-22350). Sample (30 µg of whole cell lysate). Lane A: Raji. 10% SDS PAGE. PCBP2 Polyclonal Antibody (Product # PA5-22350) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCBP2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PCBP2 Polyclonal Antibody (Product # PA5-22350) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of hnRNP E2 in methanol-fixed A431 cells using a hnRNP E2 polyclonal antibody (Product # PA5-22350) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PCBP2 Polyclonal Antibody detects PCBP2 protein at nucleus and cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PCBP2 Polyclonal Antibody (Product # PA5-22350) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PCBP2 Polyclonal Antibody detects PCBP2 protein at nucleus and cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PCBP2 Polyclonal Antibody (Product # PA5-22350) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PCBP2 was performed in Jurkat whole cell extracts using 5 µg of PCBP2 Polyclonal Antibody (Product # PA5-22350). Samples were transferred to a membrane and probed with PCBP2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
