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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX114617 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114617, RRID:AB_11171952
- Product name
- PCBP2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: NCI-H929 10% SDS PAGE GTX114617 diluted at 1:2000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCBP2 antibody detects PCBP2 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 12 % SDS-PAGE, and blotted with PCBP2 antibody (GTX114617) diluted by 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCBP2 antibody detects PCBP2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PCBP2 protein stained by PCBP2 antibody (GTX114617) diluted at 1:750.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of PCBP2 protein from Jurkat whole cell extracts using 5 £gg of PCBP2 antibody (GTX114617).Western blot analysis was performed using PCBP2 antibody (GTX114617).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human muscle, using PCBP2(GTX114617) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCBP2 antibody detects PCBP2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PCBP2 antibody (GTX114617) diluted at 1:500.
