Explore
Validate
LearnH00008625-M01
antibody from Abnova Corporation
Targeting: RFXANK
ANKRA1, BLS, F14150_1, MGC138628, RFX-B
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00008625-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008625-M01, RRID:AB_1137418
- Product name
- RFXANK monoclonal antibody (M01), clone 4G10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant RFXANK.
- Antigen sequence
MELTQPAEDLIQTQQTPASELGDPEDPGEEAADGS
DTVVLSLFPCTPEPVNPEPDASVSSPQAGSSLKHS
TTLTNRQRGNEVSALPATLD- Isotype
- IgG
- Antibody clone number
- 4G10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RFXANK expression in transfected 293T cell line by RFXANK monoclonal antibody (M01), clone 4G10.Lane 1: RFXANK transfected lysate (Predicted MW: 25.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RFXANK is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to RFXANK on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
