MA5-34620

antibody from Invitrogen Antibodies

Targeting: VIL1 D2S1471, VIL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34620

Provider

Invitrogen Antibodies

Product name

Villin Recombinant Rabbit Monoclonal Antibody (JU34-75)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: Mouse colon tissue lysate, human small intestine tissue lysate, human colon tissue lysate, rat kidney tissue lysate, Hela, HepG2, LOVO, human colon cancer tissue, human kidney, mouse colon tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JU34-75

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of villin1 in Positive control: Lane 1: Mouse colon, Lane 2: Human small intestine, Lane 3: Human colon, Lane 4: Rat kidney. Samples were incubated with villin1 monoclonal antibody (Product # MA5-34620), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of villin1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with villin1 monoclonal antibody (Product # MA5-34620), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of villin1 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with villin1 monoclonal antibody (Product # MA5-34620), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of villin1 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with villin1 monoclonal antibody (Product # MA5-34620), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of villin1 in paraffin-embedded mouse colon tissue. Samples were incubated with villin1 monoclonal antibody (Product # MA5-34620), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of villin1 in paraffin-embedded human kidney tissue. Samples were incubated with villin1 monoclonal antibody (Product # MA5-34620), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of villin1 in paraffin-embedded human colon cancer tissue. Samples were incubated with villin1 monoclonal antibody (Product # MA5-34620), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of villin1 in HeLa cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with villin1 monoclonal antibody (Product # MA5-34620) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.