GTX109723

antibody from GeneTex

Targeting: KRT7 CK7, K2C7, K7, SCL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX109723

Provider

GeneTex

Proper citation

GeneTex Cat#GTX109723, RRID:AB_1950654

Product name

Cytokeratin 7 antibody [N1C2]

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

References

Submitted references

ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.

DaSilva-Arnold SC, Kuo CY, Davra V, Remache Y, Kim PCW, Fisher JP, Zamudio S, Al-Khan A, Birge RB, Illsley NP
Molecular human reproduction 2019 Feb 1;25(2):61-75

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TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.

Pei YF, Yin XM, Liu XQ
Biochimica et biophysica acta. Molecular basis of disease 2018 Jan;1864(1):197-207

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Human trophoblast epithelial-mesenchymal transition in abnormally invasive placenta.

DaSilva-Arnold SC, Zamudio S, Al-Khan A, Alvarez-Perez J, Mannion C, Koenig C, Luke D, Perez AM, Petroff M, Alvarez M, Illsley NP
Biology of reproduction 2018 Aug 1;99(2):409-421

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Endoglin Is Essential for the Maintenance of Self-Renewal and Chemoresistance in Renal Cancer Stem Cells.

Hu J, Guan W, Liu P, Dai J, Tang K, Xiao H, Qian Y, Sharrow AC, Ye Z, Wu L, Xu H
Stem cell reports 2017 Aug 8;9(2):464-477

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate)A: A431 (GTX27909)10% SDS PAGEGTX109723 diluted at 1:10000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N1C2] (GTX109723) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N1C2] (GTX109723) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 7(GTX109723) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of human placenta (paraformaldehyde-fixed frozen sections), using Cytokeratin 7(GTX109723) antibody at 1:200 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Cytokeratin 7(GTX109723) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4¢J and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.