MAB282-100

antibody from R&D Systems

Targeting: CCL7 FIC, MARC, MCP-3, MCP3, NC28, SCYA6, SCYA7

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB282-100

Provider

R&D Systems

Product name

Human CCL7/MCP-3/MARC Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human CCL7/MCP-3/MARC in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CCL2/MCP-1, rhCCL8/MCP-2, or rhCCL13/MCP-4 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q7Z7Q8


Isotype

IgG

Antibody clone number

36320

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth.

Zhang J, Li H, Wu Q, Chen Y, Deng Y, Yang Z, Zhang L, Liu B
Redox biology 2019 Apr;22:101116

---

Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.

Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
International journal of cancer 2010 Jul 15;127(2):332-44

---

Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.

Haringman JJ, Smeets TJ, Reinders-Blankert P, Tak PP
Annals of the rheumatic diseases 2006 Mar;65(3):294-300

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Chemotaxis Induced by CCL7/MCP-3 and Neutralization by Human CCL7/MCP-3 Antibody. Recombinant Human CCL7/MCP-3 (Catalog # 282-P3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL7/MCP-3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL7/MCP-3 Monoclonal Antibody (Catalog # MAB282). The ND₅₀ is typically 1.5-4.5 µg/mL.