Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00007536-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007536-M01, RRID:AB_2239067
- Product name
- Mouse Monoclonal Splicing Factor 1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SF1 - splicing factor 1
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - SF1 monoclonal antibody (M01), clone 2E12. Analysis of SF1 expression in HeLa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01A), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Splicing Factor 1 Antibody (2E12.) [H00007536-M01] - Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Detection limit for recombinant GST tagged SF1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 transfected lysate using anti-SF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SF1 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.