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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX129604 - Provider product page
- Provider
- GeneTex
- Product name
- Timeless antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TIMELESS antibody detects TIMELESS protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract D. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGETIMELESS antibody (GTX129604) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TIMELESS antibody detects TIMELESS protein by western blot analysis.A. 30 £gg Neuro2A whole cell extract B. 30 £gg C8D30 whole cell extract C. 30 £gg NIH-3T3 whole cell extract D. 30 £gg Raw 264.7 whole cell extract E. 30 £gg C2Cl2 whole cell extract5 % SDS-PAGETIMELESS antibody (GTX129604) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TIMELESS antibody detects TIMELESS protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TIMELESS protein stained by TIMELESS antibody (GTX129604) diluted at 1:2000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of TIMELESS protein from 293T whole cell extracts using 5 £gg of TIMELESS antibody (GTX129604).Western blot analysis was performed using TIMELESS antibody (GTX129604).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
