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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00008914-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008914-D01, RRID:AB_10962924
- Product name
- TIMELESS MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human TIMELESS protein.
- Antigen sequence
MDLHMMNCELLATCSALGYLEGDTYHKEPDCLESV
KDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILT
QHHQDKPLFDAVIRLMVNLTQPALLCFGNLPKEPS
FRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYEL
LQLGWEERQEEDNLLIERILLLVRNILHVPADLDQ
EKKIDDDASAHDQLLWAIHLSGLDDLLLFLASSSA
EEQWSLHVLEIVSLMFRDQNPEQLAGVGQGRLAQE
RSADFAELEVLRQREMAEKKTRALQRGNRHSRFGG
SYIVQGLKSIGERDLIFHKGLHNLRNYSSDLGKQP
KKVPKRRQAARELSIQRRSALNVRLFLRDFCSEFL
ENCYNRLMGSVKDHLLREKAQQHDETYYMWALAFF
MAFNRAASFRPGLVSETLSVRTFHFIEQNLTNYYE
MMLTDRKEAASWARRMHLALKAYQELLATVNEMDL
SPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFD
ERCQPRSFLRDLVETTHLFLKMLERFCRSRGNLVV
QNKQKKRRKKKKKVLDQAIVSGNVPSSPEEVEAVW
PALAEQLQCCAQNSELSMDSVVPFDAASEVPVEEQ
RAEAMVRIQDCLLAGQAPQALTLLRSAREVWPEGD
VFGSQDISPEEEIQLLKQILSAPLPRQQGPEERGA
EEEEEEEEEEEEELQVVQVSEKEFNFLDYLKRFAC
STVVRAYVLLLRSYQQNSAHTNHCIVKMLHRLAHD
LKMEALLFQLSVFCLFNRLLSDPAAGAYKELVTFA
KYILGKFFALAAVNQKAFVELLFWKNTAVVREMTE
GYGSLDDRSSSRRAPTWSPEEEAHLQELYLANKDV
EGQDVVEAILAHLNTVPRTRKQIIHHLVQMGLADS
VKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDV
LGHIMKNITAKRSRARIVDKLLALGLVAERRELYK
KRQKKLASSILPNGAESLKDFCQEDLEEEENLPEE
DSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGF
SIPLLWLQNCLIRAADDREEDGCSQAVPLVPLTEE
NEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAK
LSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQG
SDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDA
VGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELG
APGIQKKKRYQIEDDEDD- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of TIMELESS expression in transfected 293T cell line (H00008914-T01) by TIMELESS MaxPab polyclonal antibody.Lane 1: TIMELESS transfected lysate(138.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to TIMELESS on HeLa cell. [antibody concentration 20 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of TIMELESS transfected lysate using anti-TIMELESS MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TIMELESS purified MaxPab mouse polyclonal antibody (B01P) (H00008914-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
