H00059067-D01P

antibody from Abnova Corporation

Targeting: IL21 IL-21, Za11

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00059067-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00059067-D01P, RRID:AB_1576305

Product name

IL21 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human IL21 protein.

Antigen sequence

MRSSPGNMERIVICLMVIFLGTLVHKSSSQGQDRH
MIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETN
CEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKR
KPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERF
KSLLQKMIHQHLSSRTHGSEDS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

IL21 MaxPab rabbit polyclonal antibody. Western Blot analysis of IL21 expression in human liver.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of IL21 expression in transfected 293T cell line (H00059067-T02) by IL21 MaxPab polyclonal antibody.Lane 1: IL21 transfected lysate(18.70 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

IL21 MaxPab rabbit polyclonal antibody. Western Blot analysis of IL21 expression in mouse kidney.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

IL21 MaxPab rabbit polyclonal antibody. Western Blot analysis of IL21 expression in A-431.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between IL21 and DGKA. HeLa cells were stained with anti-IL21 rabbit purified polyclonal 1:1200 and anti-DGKA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)