Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA325121 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal LCAT Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal LCAT Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- LCAT
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.39 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of LCAT Antibody (Center) (Cat. #TA325121) in K562 cell line lysates (35ug/lane). LCAT (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of LCAT Antibody (Center) (Cat. #TA325121) with 293 cell followed by Alexa Fluor?????488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- LCAT Antibody (Center) (Cat. #TA325121) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LCAT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- LCAT Antibody (Center) (Cat. #TA325121) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC