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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AP08748PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP08748PU-N, RRID:AB_1975714
- Product name
- anti Synaptotagmin pSer309
- Antibody type
- Polyclonal
- Antigen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser309 of Synaptotagmin.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
Submitted references Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis.
Leal-Ortiz S, Waites CL, Terry-Lorenzo R, Zamorano P, Gundelfinger ED, Garner CC
The Journal of cell biology 2008 Jun 2;181(5):831-46
The Journal of cell biology 2008 Jun 2;181(5):831-46
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k Synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. Synaptotagmin pSer309 and PSD95 Double-Label Immunostaining of 14 DIV Cortical Neurons.
