Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-56585 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56585, RRID:AB_2056731
- Product name
- Mouse Monoclonal TEM8/ANTXR1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. TEM8 is observed at ~80 kDa in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kDa and 85 kDa double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kDa band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail. Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Single-Cell Analysis Reveals Fibroblast Clusters Linked to Immunotherapy Resistance in Cancer.
Isolated tumor endothelial cells maintain specific character during long-term culture.
Anthrax toxin receptor 1/tumor endothelium marker 8 mediates cell spreading by coupling extracellular ligands to the actin cytoskeleton.
TEM8 expression stimulates endothelial cell adhesion and migration by regulating cell-matrix interactions on collagen.
TEM8 interacts with the cleaved C5 domain of collagen alpha 3(VI).
TEM8 interacts with the cleaved C5 domain of collagen alpha 3(VI).
Kieffer Y, Hocine HR, Gentric G, Pelon F, Bernard C, Bourachot B, Lameiras S, Albergante L, Bonneau C, Guyard A, Tarte K, Zinovyev A, Baulande S, Zalcman G, Vincent-Salomon A, Mechta-Grigoriou F
Cancer discovery 2020 May 20;
Cancer discovery 2020 May 20;
Isolated tumor endothelial cells maintain specific character during long-term culture.
Matsuda K, Ohga N, Hida Y, Muraki C, Tsuchiya K, Kurosu T, Akino T, Shih SC, Totsuka Y, Klagsbrun M, Shindoh M, Hida K
Biochemical and biophysical research communications 2010 Apr 16;394(4):947-54
Biochemical and biophysical research communications 2010 Apr 16;394(4):947-54
Anthrax toxin receptor 1/tumor endothelium marker 8 mediates cell spreading by coupling extracellular ligands to the actin cytoskeleton.
Werner E, Kowalczyk AP, Faundez V
The Journal of biological chemistry 2006 Aug 11;281(32):23227-36
The Journal of biological chemistry 2006 Aug 11;281(32):23227-36
TEM8 expression stimulates endothelial cell adhesion and migration by regulating cell-matrix interactions on collagen.
Hotchkiss KA, Basile CM, Spring SC, Bonuccelli G, Lisanti MP, Terman BI
Experimental cell research 2005 Apr 15;305(1):133-44
Experimental cell research 2005 Apr 15;305(1):133-44
TEM8 interacts with the cleaved C5 domain of collagen alpha 3(VI).
Nanda A, Carson-Walter EB, Seaman S, Barber TD, Stampfl J, Singh S, Vogelstein B, Kinzler KW, St Croix B
Cancer research 2004 Feb 1;64(3):817-20
Cancer research 2004 Feb 1;64(3):817-20
TEM8 interacts with the cleaved C5 domain of collagen alpha 3(VI).
Nanda A, Carson-Walter EB, Seaman S, Barber TD, Stampfl J, Singh S, Vogelstein B, Kinzler KW, St Croix B
Cancer research 2004 Feb 1;64(3):817-20
Cancer research 2004 Feb 1;64(3):817-20
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection in TEM8 transfected cell lysate using this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Human colon stained with TEM8 antibody at 2.5 ug/ml concentration using DAB chromogen detection method (10X magnification)
