OAAB05683

antibody from Aviva Systems Biology

Targeting: TNFRSF14 ATAR, CD270, HVEA, HVEM, LIGHTR, TR2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB05683

Provider

Aviva Systems Biology

Product name

TNFRSF14 antibody - center region

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of anti-TNFRSF14 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in HL-60 cell line lysate. TNFRSF14 (arrow) was detected using the purified Pab (1:250 dilution).

Sample type

HL-60 cell line lysates

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with TNFRSF14 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Sample type

human lung carcinoma

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

TNFRSF14 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Sample type

HL-60 cells

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol