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Western blot
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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-1121 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1121, RRID:AB_2219694
- Product name
- Goat Polyclonal ACADM Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize both reported isoforms.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays.
Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1.
Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1.
Rho JH, Lampe PD
Journal of proteome research 2013 May 3;12(5):2311-20
Journal of proteome research 2013 May 3;12(5):2311-20
Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1.
Matsubara Y, Kraus JP, Yang-Feng TL, Francke U, Rosenberg LE, Tanaka K
Proceedings of the National Academy of Sciences of the United States of America 1986 Sep;83(17):6543-7
Proceedings of the National Academy of Sciences of the United States of America 1986 Sep;83(17):6543-7
Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1.
Matsubara Y, Kraus JP, Yang-Feng TL, Francke U, Rosenberg LE, Tanaka K
Proceedings of the National Academy of Sciences of the United States of America 1986 Sep;83(17):6543-7
Proceedings of the National Academy of Sciences of the United States of America 1986 Sep;83(17):6543-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACADM Antibody [NB100-1121] - Analysis of ACADM in Human Heart lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 0.05 ug/mL. Primary incubated for 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACADM Antibody [NB100-1121] - Staining (0.05ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACADM Antibody [NB100-1121] - Staining of paraffin embedded human liver using this antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACADM Antibody [NB100-1121] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
