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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-15364 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Testosterone supplementation improves glucose homeostasis despite increasing hepatic insulin resistance in male mouse model of type 2 diabetes mellitus.
Pal M, Gupta S
Nutrition & diabetes 2016 Dec 12;6(12):e236
Nutrition & diabetes 2016 Dec 12;6(12):e236
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PCK1 using a PCK1 polyclonal antibody (Product # PA5-15364) in mouse liver tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a PCK1 polyclonal antibody (Product # PA5-15364) in MCF-7 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a PCK1 polyclonal antibody (Product # PA5-15364), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 ( a ) Schematic representation of HFD feeding and treatment duration in animals. ( b ) Reduced hepatic glucose output in Treated animals. ( b ) Fasting BGL of Control and Treated animals. The data were analyzed by t -test, data represent mean+-s.d., n =8, P 0.05; ( c - e ) PTT in Control and Treated animals after 4 ( c ), 16 ( d ) and 32 ( e ) weeks of treatment. The data were analyzed by t -test, data represent mean+-s.d., n =8, P 0.1. ( g - j ) Immonoblot analyses depict reduced PEPCK level, but increased insulin resistance in the liver of Treated animals. ( g ) Immunoblot and densitometry for PEPCK levels in the liver of Control and Treated animals. The data were analyzed by t -test, data represent mean+-s.d. of three independent experiments ( n =3), P
