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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- MA3-041 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Acetylcholinesterase Monoclonal Antibody (ZR3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA3-041 contains 100 µL of in vitro produced, protein A purified IgG in PBS containing 1 mg/mL BSA and 0.05% sodium azide.
- Antibody clone number
- ZR3
- Concentration
- 1.0 mg/mL
Submitted references Death of intermediolateral spinal cord neurons follows selective, complement-mediated destruction of peripheral preganglionic sympathetic terminals by acetylcholinesterase antibodies.
Paraoxon toxicity is not potentiated by prior reduction in blood acetylcholinesterase.
Monoclonal antibodies to rat brain acetylcholinesterase: comparative affinity for soluble and membrane-associated enzyme and for enzyme from different vertebrate species.
Brimijoin S, Moser V, Hammond P, Oka N, Lennon VA
Neuroscience 1993 May;54(1):201-23
Neuroscience 1993 May;54(1):201-23
Paraoxon toxicity is not potentiated by prior reduction in blood acetylcholinesterase.
Padilla S, Moser VC, Pope CN, Brimijoin WS
Toxicology and applied pharmacology 1992 Nov;117(1):110-5
Toxicology and applied pharmacology 1992 Nov;117(1):110-5
Monoclonal antibodies to rat brain acetylcholinesterase: comparative affinity for soluble and membrane-associated enzyme and for enzyme from different vertebrate species.
Rakonczay Z, Brimijoin S
Journal of neurochemistry 1986 Jan;46(1):280-7
Journal of neurochemistry 1986 Jan;46(1):280-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Mouse Brain (lane 1), HeLa (lane 2), SK-N-SH (lane 3) and U-87 MG (lane 4). The blots were probed with Anti-Acetyl cholinesterase Mouse Monoclonal Antibody (Product # MA3-041, 1-3 µg/mL) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). Three bands 67, 65 and 58 kDa corresponding to Acetyl cholinesterase was observed across cell lines tested expect HeLa. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) (Product # MA3-041) shows staining in C6 glioma cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase (Product # MA3-041) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) (Product # MA3-041) shows staining in U251 glioma cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase (Product # MA3-041) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Acetyl cholinesterase was done on U-87 MG cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Acetyl cholinesterase Mouse Monoclonal Antibody (Product # MA3-041, red histogram) or with mouse isotype control (yellow histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
