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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-81528 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUP107 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NUP107 Polyclonal Antibody (Product # PA5-81528) at 1:500 dilution. Lane A: A549 Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 106 kDa. Observed band size: 115 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of NUP107 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NUP107 Polyclonal Antibody (Product # PA5-81528, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NUP107 Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate, Lane B: 0.5 mg Hela Whole Cell Lysate 1 µL with NUP107 Polyclonal Antibody (Product # PA5-81528) and 15 µL of 50 % Protein G agarose. Primary antibody: NUP107 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 106 kDa. Observed band size: 106 kDa.
