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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-47514 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carboxypeptidase E Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) CPA1, recombinant mouse CPA4 and rhCPB1 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Carboxypeptidase E in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in Carboxypeptidase E polyclonal antibody (Product # PA5-47514) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Carboxypeptidase E in immersion fixed frozen sections of mouse embryo (9.5 d.p.c.). Samples were incubated with Carboxypeptidase E polyclonal antibody (Product # PA5-47514) using a dilution of 10 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to the developing liver.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Carboxypeptidase E in A172 human glioblastoma cell line. Samples were incubated in Carboxypeptidase E polyclonal antibody (Product # PA5-47514) or control antibody followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol and saponin.
