Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF6587 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SUMO1-Specific Peptidase 1/ SENP1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SUMO1-Specific Peptidase 1/SENP1 in direct ELISAs.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9P0U3- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SUMO-Specific Peptidase 1/SENP1 in T47D Human Cell Line. SUMO-Specific Peptidase 1/SENP1 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human SUMO-Specific Peptidase 1/SENP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6587) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SUMO1-Specific Peptidase 1/SENP1 in Human Testis. SUMO1-Specific Peptidase 1/SENP1 was detected in immersion fixed paraffin-embedded sections of human testis using Sheep Anti-Human SUMO1-Specific Peptidase 1/SENP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6587) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to late spermatids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
