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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- ARG20563 - Provider product page
- Provider
- Arigo
- Product name
- anti-IL-17A antibody
- Antibody type
- Polyclonal
- Antigen
- Highly pure (>98%) recombinant Human IL-17A.
- Description
- Affinity purification with immunogen.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 3.0 - 1.5 ng (Left to right) of recombinant Human IL-17A stained with ARG20563 anti-IL-17A antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Sandwich ELISA: ARG20563 anti-IL-17A antibody used as detection antibody results of a typical standard run with optical density reading at 405 - 650 nm.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed, paraffin-embedded sections of Human breast invasive ductal carcinoma stained with ARG20563 anti-IL-17A antibody at 0.25 µg/ml dilution (overnight incubation at 4°C). Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
