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antibody from R&D Systems
Targeting: IL17A
CTLA8, IL-17, IL-17A, IL17
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [45]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-317-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17/IL-17A Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant canine IL-17 is observed, approximately 10% cross-reactivity with recombinant human IL-17F is observed and less than 5% cross-reactivity with recombinant mouse IL-17 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q16552- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Composition and Clinical Impact of the Immunologic Tumor Microenvironment in Oral Squamous Cell Carcinoma.
Interleukin (IL)-13 and IL-17A contribute to neo-osteogenesis in chronic rhinosinusitis by inducing RUNX2.
Expression of IL-23/Th17-related cytokines in basal cell carcinoma and in the response to medical treatments.
Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation.
Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis.
Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells.
Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways.
Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.
IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy.
Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells?
Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer.
RORγt(+) hematopoietic cells are necessary for tumor cell proliferation during colitis-associated tumorigenesis in mice.
Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
IL-17/Th17 pathway is activated in acne lesions.
Allergic contact dermatitis in psoriasis patients: typical, delayed, and non-interacting.
Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway.
Sera from remitting and secondary progressive multiple sclerosis patients disrupt the blood-brain barrier.
Th1 response and systemic treg deficiency in inclusion body myositis.
Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD.
In vivo induction of cutaneous inflammation results in the accumulation of extracellular trap-forming neutrophils expressing RORγt and IL-17.
The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
IL-17A induces endothelial inflammation in systemic sclerosis via the ERK signaling pathway.
Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement.
Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
Interleukin-17 expression in neutrophils and Th17 cells in cutaneous T-cell lymphoma associated with neutrophilic infiltrate of the skin.
RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice.
Induction of endothelial nitric oxide synthase expression by IL-17 in human vascular endothelial cells: implications for vascular remodeling in transplant vasculopathy.
Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers.
Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
IL-17 induces monocyte migration in rheumatoid arthritis.
IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides.
Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum.
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
Boxberg M, Leising L, Steiger K, Jesinghaus M, Alkhamas A, Mielke M, Pfarr N, Götz C, Wolff KD, Weichert W, Kolk A
Journal of immunology (Baltimore, Md. : 1950) 2019 Jan 1;202(1):278-291
Journal of immunology (Baltimore, Md. : 1950) 2019 Jan 1;202(1):278-291
Interleukin (IL)-13 and IL-17A contribute to neo-osteogenesis in chronic rhinosinusitis by inducing RUNX2.
Khalmuratova R, Shin HW, Kim DW, Park JW
EBioMedicine 2019 Aug;46:330-341
EBioMedicine 2019 Aug;46:330-341
Expression of IL-23/Th17-related cytokines in basal cell carcinoma and in the response to medical treatments.
Pellegrini C, Orlandi A, Costanza G, Di Stefani A, Piccioni A, Di Cesare A, Chiricozzi A, Ferlosio A, Peris K, Fargnoli MC
PloS one 2017;12(8):e0183415
PloS one 2017;12(8):e0183415
Zoonotic intestinal helminths interact with the canine immune system by modulating T cell responses and preventing dendritic cell maturation.
Junginger J, Raue K, Wolf K, Janecek E, Stein VM, Tipold A, Günzel-Apel AR, Strube C, Hewicker-Trautwein M
Scientific reports 2017 Sep 4;7(1):10310
Scientific reports 2017 Sep 4;7(1):10310
Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis.
Rios DA, Valva P, Casciato PC, Frias S, Soledad Caldirola M, Gaillard MI, Bezrodnik L, Bandi J, Galdame O, Ameigeiras B, Krasniansky D, Brodersen C, Mullen E, Matteo EN, Preciado MV
Scientific reports 2017 Oct 16;7(1):13283
Scientific reports 2017 Oct 16;7(1):13283
Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells.
Seebauer CT, Brunner S, Glockzin G, Piso P, Ruemmele P, Schlitt HJ, Geissler EK, Fichtner-Feigl S, Kesselring R
Oncoimmunology 2016;5(12):e1242543
Oncoimmunology 2016;5(12):e1242543
Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways.
Fattahi F, Brandsma CA, Lodewijk M, Reinders-Luinge M, Postma DS, Timens W, Hylkema MN, Ten Hacken NH
PloS one 2016;11(8):e0161433
PloS one 2016;11(8):e0161433
Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.
Mariuzzi L, Domenis R, Orsaria M, Marzinotto S, Londero AP, Bulfoni M, Candotti V, Zanello A, Ballico M, Mimmi MC, Calcagno A, Marchesoni D, Di Loreto C, Beltrami AP, Cesselli D, Gri G
Laboratory investigation; a journal of technical methods and pathology 2016 Sep;96(9):959-971
Laboratory investigation; a journal of technical methods and pathology 2016 Sep;96(9):959-971
IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy.
Millar NL, Akbar M, Campbell AL, Reilly JH, Kerr SC, McLean M, Frleta-Gilchrist M, Fazzi UG, Leach WJ, Rooney BP, Crowe LA, Murrell GA, McInnes IB
Scientific reports 2016 Jun 6;6:27149
Scientific reports 2016 Jun 6;6:27149
Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells?
Nussbaumer-Ochsner Y, Stolk J, Ferraz da Silva LF, van Schadewijk A, de Jeu RC, Prins FA, Mauad T, Rabe KF, Hiemstra PS
PloS one 2015;10(6):e0129426
PloS one 2015;10(6):e0129426
Correlations between immune response and vascularization qRT-PCR gene expression clusters in squamous cervical cancer.
Punt S, Houwing-Duistermaat JJ, Schulkens IA, Thijssen VL, Osse EM, de Kroon CD, Griffioen AW, Fleuren GJ, Gorter A, Jordanova ES
Molecular cancer 2015 Mar 31;14:71
Molecular cancer 2015 Mar 31;14:71
RORγt(+) hematopoietic cells are necessary for tumor cell proliferation during colitis-associated tumorigenesis in mice.
Martin M, Kesselring RK, Saidou B, Brunner SM, Schiechl G, Mouris VF, Wege AK, Rümmele P, Schlitt HJ, Geissler EK, Fichtner-Feigl S
European journal of immunology 2015 Jun;45(6):1667-79
European journal of immunology 2015 Jun;45(6):1667-79
Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
Liu X, Jin H, Zhang G, Lin X, Chen C, Sun J, Zhang Y, Zhang Q, Yu J
PloS one 2014;9(9):e106834
PloS one 2014;9(9):e106834
IL-17/Th17 pathway is activated in acne lesions.
Kelhälä HL, Palatsi R, Fyhrquist N, Lehtimäki S, Väyrynen JP, Kallioinen M, Kubin ME, Greco D, Tasanen K, Alenius H, Bertino B, Carlavan I, Mehul B, Déret S, Reiniche P, Martel P, Marty C, Blume-Peytavi U, Voegel JJ, Lauerma A
PloS one 2014;9(8):e105238
PloS one 2014;9(8):e105238
Allergic contact dermatitis in psoriasis patients: typical, delayed, and non-interacting.
Quaranta M, Eyerich S, Knapp B, Nasorri F, Scarponi C, Mattii M, Garzorz N, Harlfinger AT, Jaeger T, Grosber M, Pennino D, Mempel M, Schnopp C, Theis FJ, Albanesi C, Cavani A, Schmidt-Weber CB, Ring J, Eyerich K
PloS one 2014;9(7):e101814
PloS one 2014;9(7):e101814
Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway.
Xia W, Bai J, Wu X, Wei Y, Feng S, Li L, Zhang J, Xiong G, Fan Y, Shi J, Li H
PloS one 2014;9(6):e98915
PloS one 2014;9(6):e98915
Sera from remitting and secondary progressive multiple sclerosis patients disrupt the blood-brain barrier.
Shimizu F, Tasaki A, Sano Y, Ju M, Nishihara H, Oishi M, Koga M, Kawai M, Kanda T
PloS one 2014;9(3):e92872
PloS one 2014;9(3):e92872
Th1 response and systemic treg deficiency in inclusion body myositis.
Allenbach Y, Chaara W, Rosenzwajg M, Six A, Prevel N, Mingozzi F, Wanschitz J, Musset L, Charuel JL, Eymard B, Salomon B, Duyckaerts C, Maisonobe T, Dubourg O, Herson S, Klatzmann D, Benveniste O
PloS one 2014;9(3):e88788
PloS one 2014;9(3):e88788
Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD.
Chang Y, Al-Alwan L, Alshakfa S, Audusseau S, Mogas AK, Chouiali F, Nair P, Baglole CJ, Hamid Q, Eidelman DH
Respiratory research 2014 Nov 27;15:145
Respiratory research 2014 Nov 27;15:145
In vivo induction of cutaneous inflammation results in the accumulation of extracellular trap-forming neutrophils expressing RORγt and IL-17.
Keijsers RRMC, Hendriks AGM, van Erp PEJ, van Cranenbroek B, van de Kerkhof PCM, Koenen HJPM, Joosten I
The Journal of investigative dermatology 2014 May;134(5):1276-1284
The Journal of investigative dermatology 2014 May;134(5):1276-1284
The effect of adalimumab on key drivers in the pathogenesis of psoriasis.
Hendriks AG, van der Velden HM, Wolberink EA, Seyger MM, Schalkwijk J, Zeeuwen PL, de Jong EM, Pasch MC, van Erp PE, van de Kerkhof PC
The British journal of dermatology 2014 Mar;170(3):571-80
The British journal of dermatology 2014 Mar;170(3):571-80
IL-17A induces endothelial inflammation in systemic sclerosis via the ERK signaling pathway.
Xing X, Yang J, Yang X, Wei Y, Zhu L, Gao D, Li M
PloS one 2013;8(12):e85032
PloS one 2013;8(12):e85032
Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement.
Truchetet ME, Brembilla NC, Montanari E, Lonati P, Raschi E, Zeni S, Fontao L, Meroni PL, Chizzolini C
Arthritis and rheumatism 2013 May;65(5):1347-56
Arthritis and rheumatism 2013 May;65(5):1347-56
Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
Todd J, Simpson P, Estis J, Torres V, Wub AH
Cytokine 2013 Dec;64(3):660-5
Cytokine 2013 Dec;64(3):660-5
Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A.
Lotti F, Jarrar AM, Pai RK, Hitomi M, Lathia J, Mace A, Gantt GA Jr, Sukhdeo K, DeVecchio J, Vasanji A, Leahy P, Hjelmeland AB, Kalady MF, Rich JN
The Journal of experimental medicine 2013 Dec 16;210(13):2851-72
The Journal of experimental medicine 2013 Dec 16;210(13):2851-72
Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam TV, Leypoldt F, Simova O, Thom V, Friese MA, Prinz I, Hölscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
Blood 2012 Nov 1;120(18):3793-802
Blood 2012 Nov 1;120(18):3793-802
Interleukin-17 expression in neutrophils and Th17 cells in cutaneous T-cell lymphoma associated with neutrophilic infiltrate of the skin.
Fontao L, Brembilla NC, Masouyé I, Kaya G, Prins C, Dupin N, Saurat JH, Chizzolini C, Piguet V
The British journal of dermatology 2012 Mar;166(3):687-9
The British journal of dermatology 2012 Mar;166(3):687-9
RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice.
Lapinski PE, Kwon S, Lubeck BA, Wilkinson JE, Srinivasan RS, Sevick-Muraca E, King PD
The Journal of clinical investigation 2012 Feb;122(2):733-47
The Journal of clinical investigation 2012 Feb;122(2):733-47
Induction of endothelial nitric oxide synthase expression by IL-17 in human vascular endothelial cells: implications for vascular remodeling in transplant vasculopathy.
Liu AC, Lee M, McManus BM, Choy JC
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1544-50
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1544-50
Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers.
Droeser R, Zlobec I, Kilic E, Güth U, Heberer M, Spagnoli G, Oertli D, Tapia C
BMC cancer 2012 Apr 3;12:134
BMC cancer 2012 Apr 3;12:134
Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
The British journal of dermatology 2011 Jun;164(6):1228-34
The British journal of dermatology 2011 Jun;164(6):1228-34
IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Dec 15;17(24):7529-38
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Dec 15;17(24):7529-38
Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD.
Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE
Chest 2010 Nov;138(5):1140-7
Chest 2010 Nov;138(5):1140-7
Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB
PloS one 2010 Nov 24;5(11):e14108
PloS one 2010 Nov 24;5(11):e14108
IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4880-8
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4880-8
IL-17 induces monocyte migration in rheumatoid arthritis.
Shahrara S, Pickens SR, Dorfleutner A, Pope RM
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3884-91
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3884-91
IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
The Journal of allergy and clinical immunology 2009 Jan;123(1):59-66.e4
The Journal of allergy and clinical immunology 2009 Jan;123(1):59-66.e4
Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P, Wedderburn LR
Arthritis and rheumatism 2008 Mar;58(3):875-87
Arthritis and rheumatism 2008 Mar;58(3):875-87
Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides.
Jandus C, Bioley G, Rivals JP, Dudler J, Speiser D, Romero P
Arthritis and rheumatism 2008 Aug;58(8):2307-17
Arthritis and rheumatism 2008 Aug;58(8):2307-17
Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum.
Witowski J, Ksiazek K, Warnecke C, Kuźlan M, Korybalska K, Tayama H, Wiśniewska-Elnur J, Pawlaczyk K, Trómińska J, Breborowicz A, Jörres A
Kidney international 2007 Mar;71(6):514-25
Kidney international 2007 Mar;71(6):514-25
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86
Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
The American journal of pathology 2004 Feb;164(2):409-17
The American journal of pathology 2004 Feb;164(2):409-17
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-17 by Western Blot. Western blot shows lysates of human primary naïve CD4+ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-17 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Immunoprecipitation of Human IL-17. Human IL-17 was immunoprecipitated from 100 μg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) or control antibody (Catalog # AB-108-C) overnight at 4 °C. IL-17-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-17/IL-17A in Human Tonsil. IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-17/IL-17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-17 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca2+ ionomycin for 16 hours, then stained with Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA, dark orange filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-6 Secretion Induced by IL-17 and Neutralization by Human IL-17 Antibody. Recombinant Human IL-17 (Catalog # 317-ILB) stimulates IL-6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Human IL-17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND50 is typically 0.02-0.12 µg/mL.
