Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-50350 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPM6A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous levels of total GPM6A protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.9 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPM6A was performed by loading (from left to right): Human fetal brain tissue lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a GPM6A antibody (Product # PA5-50350) at a 1/950 dilution for 1 second, followed by a secondary antibody at 1/8000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GPM6A Polyclonal Antibody, (Product # PA5-50350) and a 30 kDa band corresponding to GPM6A was observed across tissues tested except in Mouse Liver, Mouse Skeletal Muscle, Rat Liver and Rat Skeletal Muscle which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Cerebellum (Lane 2), Rat Brain (Lane 3), and Rat Cerebellum (Lane 4), Mouse Liver (Lane 5), Mouse Skeletal Muscle (Lane 6), Rat Liver (Lane 7), Rat Skeletal Muscle (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GPM6A in paraffin embedded Human brain cancer tissue using GPM6A Polyclonal Antibody (Product # PA5-50350) at a 1:40 dilution.