Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-35305 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APRT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APRT in A2058 cell lysate (35 µg/lane) using an APRT polyclonal antibody (Product # PA5-35305).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of APRT showing staining in the cytoplasm and nucleus of A549 cells using an APRT polyclonal antibody (Product # PA5-35305) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/mL, 1 h at 37ºC).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of APRT in formalin-fixed, paraffin-embedded human hepatocarcinoma using an APRT polyclonal antibody (Product # PA5-35305) followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of APRT in HepG2 cells (bottom) compared to a negative control (top) using an APRT polyclonal antibody (Product # PA5-35305) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.