Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX102144 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102144, RRID:AB_10615370
- Product name
- CIZ1 antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CIZ1 antibody [N2C1], Internal detects CIZ1 protein by Western blot analysis.A. 30 £gg HeLa whole cell lysate/extract7.5 % SDS-PAGECIZ1 antibody [N2C1], Internal (GTX102144) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CIZ1(GTX102144) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CIZ1 antibody immunoprecipitates CIZ1 protein in IP experiments. IP Sample: 1000 £gg HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of CIZ1 protein by 2.5 £gg of CIZ1 antibody (GTX102144) 7.5% SDS-PAGE The immunoprecipitated CIZ1 protein was detected by CIZ1 antibody (GTX102144) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CIZ1(GTX102144) antibody at 1:500 dilution.